USP18 ELISA Kit (Human)

43 kDa ISG15-specific protease; ISG15-specific-processing protease; ISG43; PTORCH2; ubiquitin specific protease 18; ubl carboxyl-terminal hydrolase 18; ubl thioesterase 18; ubl thiolesterase 18; UBP43.

Involved in the regulation of inflammatory response to interferon type 1 (PubMed:27325888) . Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.

Aviva Systems Biology USP18 ELISA Kit (Human) (OKWB00225) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for USP18 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for USP18 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample USP18 captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type Range Mean Serum (n=5) 88-97 92 EDTA Plasma (n=5) 85-100 93 Heparin Plasma (n=5) 88-105 98 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 88-97 92 Serum (n=5) 88-97 92 EDTA Plasma (n=5) 85-100 93 EDTA Plasma (n=5) 85-100 93 Heparin Plasma (n=5) 88-105 98 Heparin Plasma (n=5) 88-105 98

Component Amount USP18 Microplate 96 Wells (12 x 8 Well strips) USP18 Lyophilized Standard 2 x 10 ng 100X USP18 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount USP18 Microplate 96 Wells (12 x 8 Well strips) USP18 Microplate 96 Wells (12 x 8 Well strips) USP18 Lyophilized Standard 2 x 10 ng USP18 Lyophilized Standard 2 x 10 ng 100X USP18 Biotinylated Detector Anitbody 1 x 120 uL 100X USP18 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL