QTRT1 ELISA Kit (Human)

FP3235; guanine insertion enzyme; queuine tRNA-ribosyltransferase 1; queuine tRNA-ribosyltransferase catalytic subunit 1; TGT; TGT, 43-KD subunit; TGT, catalytic subunit; TGUT; tRNA-guanine transglycosylase.

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU (N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7- (((4,5-cis-dihydroxy-2-cyclopenten-1-yl) amino) methyl) -7-deazaguanosine) (PubMed:11255023, PubMed:20354154) . Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity) .

Aviva Systems Biology QTRT1 ELISA Kit (Human) (OKWB00222) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for QTRT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for QTRT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample QTRT1 captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type Range Mean Serum (n=5) 90-96 92 EDTA Plasma (n=5) 88-105 95 Heparin Plasma (n=5) 86-102 94 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 90-96 92 Serum (n=5) 90-96 92 EDTA Plasma (n=5) 88-105 95 EDTA Plasma (n=5) 88-105 95 Heparin Plasma (n=5) 86-102 94 Heparin Plasma (n=5) 86-102 94

Component Amount QTRT1 Microplate 96 Wells (12 x 8 Well strips) QTRT1 Lyophilized Standard 2 x 20 ng 100X QTRT1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount QTRT1 Microplate 96 Wells (12 x 8 Well strips) QTRT1 Microplate 96 Wells (12 x 8 Well strips) QTRT1 Lyophilized Standard 2 x 20 ng QTRT1 Lyophilized Standard 2 x 20 ng 100X QTRT1 Biotinylated Detector Anitbody 1 x 120 uL 100X QTRT1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL