BCL2L12 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision

CAS Number

7732-18-5

Gene Name

BCL2 like 12

Gene Aliases

Bcl-2 related proline-rich protein; BCL2-like 12 (proline rich) ; bcl-2-like protein 12; Bcl-2-related proline-rich protein.

Gene ID

83596

Accession Number

NP_001035758

Reactivity

Homo sapiens|Human

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric, OD 450 nm

Assay Principle

Aviva Systems Biology BCL2L12 ELISA Kit (Human) (OKWB00208) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL2L12 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCL2L12 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCL2L12 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

Detection Range

0.313-20ng/ml

Recovery

Sample Type Range Mean Serum (n=5) 88-101 92 EDTA Plasma (n=5) 94-100 98 Heparin Plasma (n=5) 85-102 97 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 88-101 92 Serum (n=5) 88-101 92 EDTA Plasma (n=5) 94-100 98 EDTA Plasma (n=5) 94-100 98 Heparin Plasma (n=5) 85-102 97 Heparin Plasma (n=5) 85-102 97

Sensitivity

0.188 ng/mL

Reconstitution

Store as indicated in product manual.

Components

Component Amount BCL2L12 Microplate 96 Wells (12 x 8 Well strips) BCL2L12 Lyophilized Standard 2 x 20 ng 100X BCL2L12 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount BCL2L12 Microplate 96 Wells (12 x 8 Well strips) BCL2L12 Microplate 96 Wells (12 x 8 Well strips) BCL2L12 Lyophilized Standard 2 x 20 ng BCL2L12 Lyophilized Standard 2 x 20 ng 100X BCL2L12 Biotinylated Detector Anitbody 1 x 120 uL 100X BCL2L12 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL