ULK1 ELISA Kit (Human)

ATG1; ATG1 autophagy related 1 homolog; ATG1A; autophagy-related protein 1 homolog; hATG1; serine/threonine-protein kinase ULK1; UNC51; Unc51.1; Unc-51-like kinase 1.

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165) .

Aviva Systems Biology ULK1 ELISA Kit (Human) (OKWB00203) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ULK1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ULK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ULK1 captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type Range Mean _x005F Serum (n=5) 86-97 92 _x005F EDTA Plasma (n=5) 90-105 98 _x005F Heparin Plasma (n=5) 87-103 96 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-97 92 Serum (n=5) 86-97 92 EDTA Plasma (n=5) 90-105 98 EDTA Plasma (n=5) 90-105 98 Heparin Plasma (n=5) 87-103 96 Heparin Plasma (n=5) 87-103 96

Component Amount _x005F ULK1 Microplate 96 Wells (12 x 8 Well strips) _x005F ULK1 Lyophilized Standard 2 x 1 ng _x005F 100X ULK1 Biotinylated Detector Anitbody 1 x 120 uL _x005F 100X Avidin-HRP Conjugate 1 x 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 25X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 10 mL _x005F Stop Solution 1 x 10 mL Component Amount Component Amount ULK1 Microplate 96 Wells (12 x 8 Well strips) ULK1 Microplate 96 Wells (12 x 8 Well strips) ULK1 Lyophilized Standard 2 x 1 ng ULK1 Lyophilized Standard 2 x 1 ng 100X ULK1 Biotinylated Detector Anitbody 1 x 120 uL 100X ULK1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL