TRH ELISA Kit (Human)

Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. May promote hair shaft elongation, prolonge the hair cycle growth phase (anagen) and antagonized its termination by TGFB2. May also increase proliferation and inhibited apoptosis of hair matrix keratinocytes.

Aviva Systems Biology TRH ELISA Kit (Human) (OKWB00188) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with TRH. Sample or standards are added to the wells along with a TRH biotinylated detector antibody and incubated. The TRH found in the sample or standards inhibits the TRH detector antibody from binding to the immobilized TRH on the plate. Excess unbound sample or standard TRH and detector antibody is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of TRH biotinylated detector antibody bound in the well and inversely proportional to the amount of TRH which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type Range Mean Serum (n=5) 91-104 99 EDTA Plasma (n=5) 85-101 91 Heparin Plasma (n=5) 87-101 93 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 91-104 99 Serum (n=5) 91-104 99 EDTA Plasma (n=5) 85-101 91 EDTA Plasma (n=5) 85-101 91 Heparin Plasma (n=5) 87-101 93 Heparin Plasma (n=5) 87-101 93

Component Amount TRH Microplate 96 Wells (12 x 8 Well strips) TRH Lyophilized Standard 2 x 500 pg 100X TRH Biotinylated Detector Anitbody 1 x 60 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount TRH Microplate 96 Wells (12 x 8 Well strips) TRH Microplate 96 Wells (12 x 8 Well strips) TRH Lyophilized Standard 2 x 500 pg TRH Lyophilized Standard 2 x 500 pg 100X TRH Biotinylated Detector Anitbody 1 x 60 uL 100X TRH Biotinylated Detector Anitbody 1 x 60 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL