SPTAN1 ELISA Kit (Human)

Alpha-fodrin; alpha-II spectrin; DEE5; EIEE5; epididymis secretory sperm binding protein; fodrin alpha chain; NEAS; spectrin alpha chain, non-erythrocytic 1; spectrin, non-erythroid alpha chain; spectrin, non-erythroid alpha subunit; SPTA2.

Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.

Aviva Systems Biology SPTAN1 ELISA Kit (Human) (OKWB00187) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SPTAN1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SPTAN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SPTAN1 captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type Range Mean Serum (n=5) 87-98 94 EDTA Plasma (n=5) 85-101 94 Heparin Plasma (n=5) 93-103 99 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 87-98 94 Serum (n=5) 87-98 94 EDTA Plasma (n=5) 85-101 94 EDTA Plasma (n=5) 85-101 94 Heparin Plasma (n=5) 93-103 99 Heparin Plasma (n=5) 93-103 99

Component Amount SPTAN1 Microplate 96 Wells (12 x 8 Well strips) SPTAN1 Lyophilized Standard 2 x 10 ng 100X SPTAN1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount SPTAN1 Microplate 96 Wells (12 x 8 Well strips) SPTAN1 Microplate 96 Wells (12 x 8 Well strips) SPTAN1 Lyophilized Standard 2 x 10 ng SPTAN1 Lyophilized Standard 2 x 10 ng 100X SPTAN1 Biotinylated Detector Anitbody 1 x 120 uL 100X SPTAN1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL