PRKAR2A ELISA Kit (Human)

DJ680N4.3; G protein-coupled receptor 73-like 1; GPR73b; GPR73L1; GPRg2; G-protein coupled receptor 73-like 1; G-protein coupled receptor I5E; HH3; KAL3; PKR2; PK-R2; prokineticin receptor 2.

Receptor for prokineticin 2. Exclusively coupled to the G (q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase.

Aviva Systems Biology PRKAR2A ELISA Kit (Human) (OKWB00182) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRKAR2A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRKAR2A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRKAR2A captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type Range Mean Serum (n=5) 85-96 91 EDTA Plasma (n=5) 86-95 92 Heparin Plasma (n=5) 91-101 96 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 85-96 91 Serum (n=5) 85-96 91 EDTA Plasma (n=5) 86-95 92 EDTA Plasma (n=5) 86-95 92 Heparin Plasma (n=5) 91-101 96 Heparin Plasma (n=5) 91-101 96

Component Amount PRKAR2A Microplate 96 Wells (12 x 8 Well strips) PRKAR2A Lyophilized Standard 2 x 20 ng 100X PRKAR2A Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount PRKAR2A Microplate 96 Wells (12 x 8 Well strips) PRKAR2A Microplate 96 Wells (12 x 8 Well strips) PRKAR2A Lyophilized Standard 2 x 20 ng PRKAR2A Lyophilized Standard 2 x 20 ng 100X PRKAR2A Biotinylated Detector Anitbody 1 x 120 uL 100X PRKAR2A Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL