What is the catalog number for DHPS ELISA Kit (Human)?

The catalog number for DHPS ELISA Kit (Human) is OKWB00169-96W.

What sizes are available for DHPS ELISA Kit (Human)?

DHPS ELISA Kit (Human) is available in the following size(s): 96 Well.

Is DHPS ELISA Kit (Human) in stock?

DHPS ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

DHPS ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision

CAS Number

7732-18-5

Gene Aliases

DS, DHS, MIG13, NEDSSWI

Gene ID

1725

Accession Number

NP_001193903

Reactivity

Human

Target

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology DHPS ELISA Kit (Human) (OKWB00169) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DHPS has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DHPS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DHPS captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

Detection Range

0.313 - 20 ng/mL

Recovery

Sample Type Range Mean Serum (n=5) 87-99 92 EDTA Plasma (n=5) 86-105 96 Heparin Plasma (n=5) 85-104 95 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 87-99 92 Serum (n=5) 87-99 92 EDTA Plasma (n=5) 86-105 96 EDTA Plasma (n=5) 86-105 96 Heparin Plasma (n=5) 85-104 95 Heparin Plasma (n=5) 85-104 95