AP2S1 ELISA Kit (Human)

Adaptor protein complex AP-2 subunit sigma; adaptor related protein complex 2 sigma 1 subunit; Adaptor-related protein complex 2 subunit sigma; AP17; AP-2 complex subunit sigma; CLAPS2; clathrin assembly protein 2 sigma small chain; clathrin coat assembly protein AP17; clathrin coat-associated protein AP17; clathrin-associated/assembly/adaptor protein, small 2 (17kD) ; FBH3; FBHOk; HA2 17 kDa subunit; HHC3; plasma membrane adaptor AP-2 17 kDa protein; sigma-2; sigma2-adaptin.

Component of the adaptor protein complex 2 (AP-2) . Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity) . May also play a role in extracellular calcium homeostasis.

Aviva Systems Biology AP2S1 ELISA Kit (Human) (OKWB00165) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with AP2S1. Sample or standards are added to the wells along with a AP2S1 biotinylated detector antibody and incubated. The AP2S1 found in the sample or standards inhibits the AP2S1 detector antibody from binding to the immobilized AP2S1 on the plate. Excess unbound sample or standard AP2S1 and detector antibody is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of AP2S1 biotinylated detector antibody bound in the well and inversely proportional to the amount of AP2S1 which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type Range Mean Serum (n=5) 87-102 94 EDTA Plasma (n=5) 88-100 95 Heparin Plasma (n=5) 85-105 92 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 87-102 94 Serum (n=5) 87-102 94 EDTA Plasma (n=5) 88-100 95 EDTA Plasma (n=5) 88-100 95 Heparin Plasma (n=5) 85-105 92 Heparin Plasma (n=5) 85-105 92

Component Amount AP2S1 Microplate 96 Wells (12 x 8 Well strips) AP2S1 Lyophilized Standard 2 x 10 ng 100X AP2S1 Biotinylated Detector Anitbody 1 x 60 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount AP2S1 Microplate 96 Wells (12 x 8 Well strips) AP2S1 Microplate 96 Wells (12 x 8 Well strips) AP2S1 Lyophilized Standard 2 x 10 ng AP2S1 Lyophilized Standard 2 x 10 ng 100X AP2S1 Biotinylated Detector Anitbody 1 x 60 uL 100X AP2S1 Biotinylated Detector Anitbody 1 x 60 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL