What is the catalog number for GPRIN1 ELISA Kit (Human)?

The catalog number for GPRIN1 ELISA Kit (Human) is OKWB00158-96W.

What sizes are available for GPRIN1 ELISA Kit (Human)?

GPRIN1 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is GPRIN1 ELISA Kit (Human) in stock?

GPRIN1 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

GPRIN1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision

CAS Number

7732-18-5

Gene Name

G protein regulated inducer of neurite outgrowth 1

Gene Aliases

G protein-regulated inducer of neurite outgrowth 1; GRIN1.

Gene ID

114787

Accession Number

NP_443131

Reactivity

Homo sapiens|Human

Target

May be involved in neurite outgrowth.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric, OD 450 nm

Assay Principle

Aviva Systems Biology GPRIN1 ELISA Kit (Human) (OKWB00158) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GPRIN1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GPRIN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GPRIN1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

Detection Range

0.156-10ng/ml

Recovery

Sample Type Range Mean Serum (n=5) 89-101 95 EDTA Plasma (n=5) 87-101 91 Heparin Plasma (n=5) 85-105 97 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 89-101 95 Serum (n=5) 89-101 95 EDTA Plasma (n=5) 87-101 91 EDTA Plasma (n=5) 87-101 91 Heparin Plasma (n=5) 85-105 97 Heparin Plasma (n=5) 85-105 97

Sensitivity

0.094 ng/mL

Reconstitution

Store as indicated in product manual.

Components

Component Amount GPRIN1 Microplate 96 Wells (12 x 8 Well strips) GPRIN1 Lyophilized Standard 2 x 10 ng 100X GPRIN1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount GPRIN1 Microplate 96 Wells (12 x 8 Well strips) GPRIN1 Microplate 96 Wells (12 x 8 Well strips) GPRIN1 Lyophilized Standard 2 x 10 ng GPRIN1 Lyophilized Standard 2 x 10 ng 100X GPRIN1 Biotinylated Detector Anitbody 1 x 120 uL 100X GPRIN1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL