TLR2-1 ELISA Kit (Chicken)
Product Specifications
Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Aliases
TLR2, TLR2-1, chTLR2
Gene ID
374141
Accession Number
NP_989609
Reactivity
Chicken
Target
Participates in the innate immune response to microbial agents. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Tlr2-1 ELISA Kit (Chicken) (OKWB00129) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tlr2-1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tlr2-1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tlr2-1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
0.313 - 20 ng/mL
Recovery
Sample Type Range Mean Serum (n=5) 95-99 97 EDTA Plasma (n=5) 87-102 96 Heparin Plasma (n=5) 91-101 95 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 95-99 97 Serum (n=5) 95-99 97 EDTA Plasma (n=5) 87-102 96 EDTA Plasma (n=5) 87-102 96 Heparin Plasma (n=5) 91-101 95 Heparin Plasma (n=5) 91-101 95
Sensitivity
0.188 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
TLR2-1
Protein Name
Toll-like receptor 2 type-1
Gene Name URL
TLR2-1
Nucleotide Accession Number
NM_204278
Frequently Asked Questions
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