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TLR2-1 ELISA Kit (Chicken)

Product Specifications

Reproducibility

Mean Inter-Assay Precision

CAS Number

7732-18-5

Gene Aliases

TLR2, TLR2-1, chTLR2

Gene ID

374141

Accession Number

NP_989609

Reactivity

Chicken

Target

Participates in the innate immune response to microbial agents. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Tlr2-1 ELISA Kit (Chicken) (OKWB00129) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tlr2-1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tlr2-1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tlr2-1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

Detection Range

0.313 - 20 ng/mL

Recovery

Sample Type Range Mean Serum (n=5) 95-99 97 EDTA Plasma (n=5) 87-102 96 Heparin Plasma (n=5) 91-101 95 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 95-99 97 Serum (n=5) 95-99 97 EDTA Plasma (n=5) 87-102 96 EDTA Plasma (n=5) 87-102 96 Heparin Plasma (n=5) 91-101 95 Heparin Plasma (n=5) 91-101 95

Sensitivity

0.188 ng/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

TLR2-1

Protein Name

Toll-like receptor 2 type-1

Gene Name URL

TLR2-1

Nucleotide Accession Number

NM_204278

Frequently Asked Questions

More Discoveries

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