DLA-64 ELISA Kit (Canine)
Selected Variant
OKWB00118-96W
Size: 96 Well
Product Specifications
Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Reactivity
Canine
Target
Involved in the presentation of foreign antigens to the immune system.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Dla-64 ELISA Kit (Canine) (OKWB00118) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dla-64 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dla-64 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dla-64 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
0.781 - 50 ng/mL
Recovery
Sample Type Range Mean Serum (n=5) 88-103 98 EDTA Plasma (n=5) 85-101 97 Heparin Plasma (n=5) 86-102 94 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 88-103 98 Serum (n=5) 88-103 98 EDTA Plasma (n=5) 85-101 97 EDTA Plasma (n=5) 85-101 97 Heparin Plasma (n=5) 86-102 94 Heparin Plasma (n=5) 86-102 94
Sensitivity
0.469 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
DLA-64
Protein Name
MHC class I antigen
Gene Name URL
DLA-64
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