LF ELISA Kit (Bovine)
Product Specifications
Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Aliases
Lf
Gene ID
280846
Accession Number
NP_851341
Reactivity
Bovine
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Lf ELISA Kit (Bovine) (OKWB00114) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lf has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lf captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
1.563 - 100 ng/mL
Recovery
Sample Type Range Mean Serum (n=5) 86-101 92 EDTA Plasma (n=5) 88-104 94 Heparin Plasma (n=5) 86-97 92 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-101 92 Serum (n=5) 86-101 92 EDTA Plasma (n=5) 88-104 94 EDTA Plasma (n=5) 88-104 94 Heparin Plasma (n=5) 86-97 92 Heparin Plasma (n=5) 86-97 92
Sensitivity
0.938 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
LF
Protein Name
Lactoferrin
Gene Name URL
LF
Nucleotide Accession Number
NM_180998
Frequently Asked Questions
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