BOLA-DQA2 ELISA Kit (Bovine)

Aviva Systems Biology Bola-dqa2 ELISA Kit (Bovine) (OKWB00105) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bola-dqa2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bola-dqa2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bola-dqa2 captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type Range Mean Serum (n=5) 85-105 95 EDTA Plasma (n=5) 87-99 91 Heparin Plasma (n=5) 89-105 95 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 85-105 95 Serum (n=5) 85-105 95 EDTA Plasma (n=5) 87-99 91 EDTA Plasma (n=5) 87-99 91 Heparin Plasma (n=5) 89-105 95 Heparin Plasma (n=5) 89-105 95

Component Amount Bola-dqa2 Microplate 96 Wells (12 x 8 Well strips) Bola-dqa2 Lyophilized Standard 2 x 100 ng 100X Bola-dqa2 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Bola-dqa2 Microplate 96 Wells (12 x 8 Well strips) Bola-dqa2 Microplate 96 Wells (12 x 8 Well strips) Bola-dqa2 Lyophilized Standard 2 x 100 ng Bola-dqa2 Lyophilized Standard 2 x 100 ng 100X Bola-dqa2 Biotinylated Detector Anitbody 1 x 120 uL 100X Bola-dqa2 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL