Hantavirus IgM ELISA Kit (Human)

Metric Sample Type n= CV% Intra-Assay Positive Serum 24 1.283 0.0568 Inter-Assay Positive Serum 24 38.31 0.1228 Inter-Assay Positive Serum 24 38.14 0.1175 Inter-Assay Positive Serum 24 25.52 0.1397 Intra-Assay Negative Serum 24 0.248 0.0711 Intra-Assay Negative Serum 24 0.244 0.0437 Intra-Assay Positive Serum 24 0.498 0.041 Metric Sample Type n= CV% Metric Sample Type n= CV% Intra-Assay Positive Serum 24 1.283 0.0568 Intra-Assay Positive Serum 24 1.283 0.0568 Inter-Assay Positive Serum 24 38.31 0.1228 Inter-Assay Positive Serum 24 38.31 0.1228 Inter-Assay Positive Serum 24 38.14 0.1175 Inter-Assay Positive Serum 24 38.14 0.1175 Inter-Assay Positive Serum 24 25.52 0.1397 Inter-Assay Positive Serum 24 25.52 0.1397 Intra-Assay Negative Serum 24 0.248 0.0711 Intra-Assay Negative Serum 24 0.248 0.0711 Intra-Assay Negative Serum 24 0.244 0.0437 Intra-Assay Negative Serum 24 0.244 0.0437 Intra-Assay Positive Serum 24 0.498 0.041 Intra-Assay Positive Serum 24 0.498 0.041

Hantaviruses are negative sense RNA viruses in the Bunyaviridae family. Humans may be infected with Hantaviruses through urine, saliva or contact with rodent waste products. Some Hantaviruses cause potentially fatal diseases in humans, such as hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS), but others have not been associated with human disease. Human infections of Hantaviruses have almost entirely been linked to human contact with rodent excrement, but recent human-to-human transmission has been reported with the Andes virus in South America. Hantavirus has an incubation time of two to four weeks in humans before symptoms of infection occur. The symptoms of HFRS can be split into five phases: • Febrile phase: Symptoms include fever, chills, sweaty palms, diarrhea, malaise, headaches, nausea, abdominal and back pain, respiratory problems such as the ones common in influenza virus infection, as well as gastro-intestinal problems. These symptoms normally occur for three to seven days and arise about two to three weeks after exposure. • Hypotensive phase: This occurs when the blood platelet levels drop and symptoms can lead to tachycardia and hypoxemia. This phase can last for 2 days. • Oliguric phase: This phase lasts for three to seven days and is characterized by the onset of renal failure and proteinuria occurs. • Diuretic phase: This is characterized by diuresis of three to six liters per day, which can last for a couple of days up to weeks. • Convalescent phase: This is normally when recovery occurs and symptoms begin to improve. Regions especially affected by HFRS include China, the Korean Peninsula, Russia (Hantaan, Puumala and Seoul viruses), and northern and western Europe (Puumala and Dobrava virus)

Aviva Systems Biology Hantavirus IgM ELISA Kit (Human) (OKNX00139) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Hantavirus antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for Human IgM is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is qualitatively proportional to the amount of sample anti-Hantavirus IgM captured the in well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol