Prealbumin ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Transthyretin

Gene Aliases

TT, Lr1, Tbpa

Gene ID

24856

Reactivity

Rat

Target

Binds thyroxine (T4) and 3,5,3' triiodothyronine (T3) ; plays a role in thyroid hormone transport in serum.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric, OD 450 nm

Assay Principle

Aviva Systems Biology Prealbumin ELISA Kit (Rat) (OKIA00159) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Transthyretin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Transthyretin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Transthyretin captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.625 ng/ml - 500 ng/ml

Sensitivity

4.508 ng/ml

Homology

Rat

Format

In Solution

Reconstitution

Store as indicated in product manual.

Components

Component Amount Anti-Rat Prealbumin (Transthyretin) Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Rat Prealbumin (Transthyretin) Calibrator 1 Component Amount Component Amount Anti-Rat Prealbumin (Transthyretin) Microplate 96 Wells (12 x 8 Well Strips) Anti-Rat Prealbumin (Transthyretin) Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Stop Solution 12 mL Rat Prealbumin (Transthyretin) Calibrator 1 Rat Prealbumin (Transthyretin) Calibrator 1