What is the catalog number for KIM-1 ELISA Kit (Rat)?

The catalog number for KIM-1 ELISA Kit (Rat) is OKIA00155-96W.

What sizes are available for KIM-1 ELISA Kit (Rat)?

KIM-1 ELISA Kit (Rat) is available in the following size(s): 1x 96 Well Plate.

Is KIM-1 ELISA Kit (Rat) in stock?

KIM-1 ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

KIM-1 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Hepatitis A virus cellular receptor 1

Gene Aliases

Kim1, KIM-1

Gene ID

286934

Reactivity

Rat

Target

May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric, OD450 nm

Assay Principle

Aviva Systems Biology KIM-1 ELISA Kit (Rat) (OKIA00155) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Hepatitis A virus cellular receptor 1 homolog has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Hepatitis A virus cellular receptor 1 homolog - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hepatitis A virus cellular receptor 1 homolog captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.1 ng/ml - 10 ng/ml

Sensitivity

0.039 ng/ml

Homology

Rat

Format

In Solution

Reconstitution

Store as indicated in product manual.

Components

Component Amount Anti-Rat KIM-1 Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Rat KIM-1 Calibrator 1 Component Amount Component Amount Anti-Rat KIM-1 Microplate 96 Wells (12 x 8 Well Strips) Anti-Rat KIM-1 Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Stop Solution 12 mL Rat KIM-1 Calibrator 1 Rat KIM-1 Calibrator 1