Prealbumin ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Transthyretin

Gene Aliases

CTS, TTN, ATTR, CTS1, PALB, TBPA, HEL111, HsT2651

Gene ID

7276

Reactivity

Human

Target

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric, OD 450 nm

Assay Principle

Aviva Systems Biology Prealbumin ELISA Kit (Human) (OKIA00081) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Transthyretin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Transthyretin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Transthyretin captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

3.125 ng/ml - 100 ng/ml

Sensitivity

0.415 ng/ml

Homology

Human

Format

In Solution

Reconstitution

Store as indicated in product manual.

Components

Component Amount _x005F Anti-Human Prealbumin (Transthyretin) Microplate 96 Wells (12 x 8 Well Strips) _x005F 20X Diluent Concentrate 50 mL _x005F 20X Wash Solution 50 mL _x005F 100X Enzyme-Antibody Conjugate 150 uL _x005F Chromogen-Substrate Solution 12 mL _x005F Stop Solution 12 mL _x005F Human Prealbumin (Transthyretin) Calibrator 1 _x005F Component Amount Component Amount Anti-Human Prealbumin (Transthyretin) Microplate 96 Wells (12 x 8 Well Strips) Anti-Human Prealbumin (Transthyretin) Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Stop Solution 12 mL Human Prealbumin (Transthyretin) Calibrator 1 Human Prealbumin (Transthyretin) Calibrator 1