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Alpha 1-Acid Glycoprotein ELISA Kit (Chicken)

Product Specifications

CAS Number

7732-18-5

Gene Name

Orosomucoid 1 (ovoglycoprotein)

Gene Aliases

OGCHI

Gene ID

395220

Reactivity

Chicken

Target

Functions as transport protein in the blood stream.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric, OD 450 nm

Assay Principle

Aviva Systems Biology Alpha 1-Acid Glycoprotein ELISA Kit (Chicken) (OKIA00015) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Alpha-1-acid glycoprotein has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Alpha-1-acid glycoprotein - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alpha-1-acid glycoprotein captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

6.25 ng/ml - 200 ng/ml

Sensitivity

1.975 ng/ml

Homology

Chicken

Format

In Solution

Reconstitution

Store as indicated in product manual.

Components

Component Amount Anti-Chicken Alpha 1-Acid Glycoprotein Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Chicken Alpha 1-Acid Glycoprotein Calibrator 1 Component Amount Component Amount Anti-Chicken Alpha 1-Acid Glycoprotein Microplate 96 Wells (12 x 8 Well Strips) Anti-Chicken Alpha 1-Acid Glycoprotein Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Stop Solution 12 mL Chicken Alpha 1-Acid Glycoprotein Calibrator 1 Chicken Alpha 1-Acid Glycoprotein Calibrator 1

Notes

Formerly GWB-374Z11

NCBI Gene Symbol

ORM1

Protein Name

Alpha-1-acid glycoprotein

Gene Name URL

ORM1

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