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CCNE1 ELISA Kit (Rat)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Aliases

Ccne, CYCLE, G1/S-specific cyclin-E1

Reactivity

Rat

Target

Essential for the control of the cell cycle at the G1/S (start) transition.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Ccne1 ELISA Kit (Rat) (OKEI00883) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccne1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccne1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Ccne1

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Biological Fluids

Detection Range

0.031 - 2 ng/mL

Sensitivity

18.75 pg/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

CCNE1

Protein Name

G1/S-specific cyclin-E1

Gene Name URL

CCNE1

Frequently Asked Questions

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