SDF4 ELISA Kit (Rat)
Product Specifications
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Aliases
45 kDa calcium-binding protein, Cab45, SDF-4, Stromal cell-derived factor 4
Gene ID
155173
Accession Number
NP_569096
Reactivity
Rat
Target
A membrane-associated isoform may be involved in the exocytosis of zymogens by pancreatic acini. May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Sdf4 ELISA Kit (Rat) (OKEI00846) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sdf4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Sdf4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Sdf4
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Biological Fluids
Detection Range
0.016 - 1 ng/mL
Sensitivity
9.375 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
SDF4
Protein Name
45 kDa calcium-binding protein
Gene Name URL
SDF4
Nucleotide Accession Number
NM_130412
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