OSTF1 ELISA Kit (Rat)
Product Specifications
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Aliases
OSF, SH3P2, bA235O14.1
Gene ID
26578
Accession Number
NP_036515
Reactivity
Rat
Target
Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor (s) enhancing osteoclast formation and activity.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Ostf1 ELISA Kit (Rat) (OKEI00806) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ostf1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ostf1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Ostf1
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Biological Fluids
Detection Range
0.156 - 10 ng/mL
Sensitivity
0.094 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
OSTF1
Protein Name
Osteoclast-stimulating factor 1
Gene Name URL
OSTF1
Nucleotide Accession Number
NM_012383
Curated Selection
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