GRIK2 ELISA Kit (Rat)
Product Specifications
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Aliases
GluK2, GluR6
Gene ID
54257
Accession Number
NP_062182
Reactivity
Rat
Target
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist . May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Grik2 ELISA Kit (Rat) (OKEI00777) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Grik2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Grik2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Grik2
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Biological Fluids
Detection Range
1.563 - 100 ng/mL
Sensitivity
0.938 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
GRIK2
Protein Name
Glutamate receptor ionotropic, kainate 2
Gene Name URL
GRIK2
Nucleotide Accession Number
NM_019309
Frequently Asked Questions
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