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CKLF ELISA Kit (Mouse)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Aliases

C32, CKLF1, CKLF3, CKLF4, CKLF5, Cklf2, Cklf6, UCK-1, HSPC224, 1700001C14Rik, 1810018M11Rik, A730028G07Rik

Gene ID

75458

Accession Number

NP_001032930

Reactivity

Mouse

Target

May play an important role in inflammation and regeneration of skeletal muscle.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Cklf ELISA Kit (Mouse) (OKEI00404) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cklf has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cklf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Cklf

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Biological Fluids

Detection Range

0.031 - 2 ng/mL

Sensitivity

18.75 pg/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

CKLF

Protein Name

Chemokine-like factor

Gene Name URL

CKLF

Nucleotide Accession Number

NM_001037841

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