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MIP ELISA Kit (Mouse)

Selected Variant

OKEI00356-96W

Size: 96 Well

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Aliases

Aq, Ct, Cat, Cts, Hfi, Lop, Svl, Aqp0, MP26, shri, MIP26, shrivelled

Gene ID

17339

Accession Number

NP_032626

Reactivity

Mouse

Target

Water channel. Channel activity is down-regulated by CALM when cytoplasmic Ca2+ levels are increased. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Mip ELISA Kit (Mouse) (OKEI00356) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mip has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mip is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Mip

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Biological Fluids

Detection Range

0.781 - 50 ng/mL

Sensitivity

0.469 ng/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

MIP

Protein Name

Lens fiber major intrinsic protein

Gene Name URL

MIP

Nucleotide Accession Number

NM_008600

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