B3GLCT ELISA Kit (Human)
Product Specifications
Reproducibility
Inter-Assay CV
Gene Aliases
B3GTL, Gal-T, B3GALTL, B3Glc-T, beta3Glc-T
Gene ID
145173
Accession Number
NP_919299
Reactivity
Human
Target
O-glucosyltransferase that transfers glucose toward fucose with a beta-1,3 linkage. Specifically glucosylates O-linked fucosylglycan on TSP type-1 domains of proteins, thereby contributing to elongation of O-fucosylglycan.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology B3GLCT ELISA Kit (Human) (OKEI00318) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for B3GLCT has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for B3GLCT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample B3GLCT
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Biological Fluids
Detection Range
0.078 - 5 ng/mL
Sensitivity
46.9 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
B3GLCT
Protein Name
Beta-1,3-glucosyltransferase
Gene Name URL
B3GLCT
CAS Number
7732-18-5
Nucleotide Accession Number
NM_194318
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