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SAP25 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene ID

100316904

Accession Number

NP_001335606

Reactivity

Human

Target

Involved in the transcriptional repression mediated by the mSIN3A but not the N-CoR corepressor complex.By similarity

Type

ELISA Kit

Assay Principle

Aviva Systems Biology SAP25 ELISA Kit (Human) (OKEI00275) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SAP25 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SAP25 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample SAP25

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Biological Fluids

Detection Range

0.031 - 2 ng/mL

Sensitivity

18.75 pg/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

SAP25

Protein Name

Histone deacetylase complex subunit SAP25

Gene Name URL

SAP25

Nucleotide Accession Number

NM_001348677

Frequently Asked Questions

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