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POU2F1 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Aliases

OCT1, OTF1, Oct1Z, oct-1B

Gene ID

5451

Accession Number

NP_001185712

Reactivity

Human

Target

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology POU2F1 ELISA Kit (Human) (OKEI00188) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for POU2F1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for POU2F1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample POU2F1

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Biological Fluids

Detection Range

0.156 - 10 ng/mL

Sensitivity

0.094 ng/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

POU2F1

Protein Name

POU domain, class 2, transcription factor 1

Gene Name URL

POU2F1

Nucleotide Accession Number

NM_001198783

Frequently Asked Questions

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