What is the catalog number for CENPA ELISA Kit (Human)?

The catalog number for CENPA ELISA Kit (Human) is OKEI00148-96W.

What sizes are available for CENPA ELISA Kit (Human)?

CENPA ELISA Kit (Human) is available in the following size(s): 96 Well.

Is CENPA ELISA Kit (Human) in stock?

CENPA ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.

Is this product species cross-reactive?

Cross-reactivity data, based on available testing, is listed under the reactivity section of the datasheet. Contact us if you need reactivity confirmed for a species not listed.

CENPA ELISA Kit (Human)

Selected Variant

OKEI00148-96W

Size: 96 Well

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Aliases

CenH3, CENP-A

Gene ID

1058

Accession Number

NP_001035891

Reactivity

Human

Target

Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes . Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore . The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 . May serve as an epigenetic mark that propagates centromere identity through replication and cell division . Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis .

Type

ELISA Kit

Assay Principle

Aviva Systems Biology CENPA ELISA Kit (Human) (OKEI00148) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CENPA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CENPA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample CENPA

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Biological Fluids

Detection Range

0.313 - 20 ng/mL

Sensitivity

0.188 ng/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

CENPA

Protein Name

Histone H3-like centromeric protein A

Gene Name URL

CENPA

Nucleotide Accession Number

NM_001042426

Price

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