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CEBPD ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Aliases

CELF, CRP3, C/EBP-delta, NF-IL6-beta

Gene ID

1052

Accession Number

NP_005186

Reactivity

Human

Target

Transcription activator that recognizes two different DNA motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers . Important transcription factor regulating the expression of genes involved in immune and inflammatory responses . Transcriptional activator that enhances IL6 transcription alone and as heterodimer with CEBPB .

Type

ELISA Kit

Assay Principle

Aviva Systems Biology CEBPD ELISA Kit (Human) (OKEI00146) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CEBPD has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CEBPD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample CEBPD

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Biological Fluids

Detection Range

3.125 - 200 ng/mL

Sensitivity

1.875 ng/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

CEBPD

Protein Name

CCAAT/enhancer-binding protein delta

Gene Name URL

CEBPD

Nucleotide Accession Number

NM_005195

Frequently Asked Questions

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