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GPX4 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Glutathione peroxidase 4

Gene Aliases

Glutathione peroxidase 4; GPx-4; GSHPx-4; mtPHG; mtPHGPx; PHG; PHGPx; phospholipid hydroperoxide glutathione peroxidase; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxide glutathione peroxidase, nuclear; sn; snGPx; sperm nuclei glutathione peroxidase.

Gene ID

625249

Accession Number

NP_001032830.2

Reactivity

Mouse, Mus musculus

Target

Phospholipid hydroperoxide glutathione peroxidase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology GPX4 ELISA Kit (Mouse) (OKEH08828) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GPX4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GPX4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GPX4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

31.2-2000 pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 114% (n = 20)

Sensitivity

10 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Phospholipid hydroperoxide glutathione peroxidase

NCBI Gene Symbol

Gpx4

Protein Name

O70325

Gene Name URL

Gpx4

Nucleotide Accession Number

NM_001037741.3

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