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APPBP2 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Amyloid beta precursor protein binding protein 2

Gene Aliases

Amyloid beta precursor protein (cytoplasmic tail) binding protein 2; Amyloid beta precursor protein-binding protein 2; amyloid protein-binding protein 2; APP-BP2; PAT1; protein interacting with APP tail 1.

Gene ID

303396

Accession Number

NP_001094439.1

Reactivity

Rat, Rattus norvegicus

Target

Amyloid protein-binding protein 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology APPBP2 ELISA Kit (Rat) (OKEH08822) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for APPBP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for APPBP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample APPBP2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86% (n = 20)

Sensitivity

0.068ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Amyloid protein-binding protein 2

NCBI Gene Symbol

Appbp2

Protein Name

A5HK05

Gene Name URL

Appbp2

Nucleotide Accession Number

NM_001100969.1

Frequently Asked Questions

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