CSF2RB ELISA Kit (Human)
Product Specifications
Reproducibility
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CAS Number
7732-18-5
Gene Name
Colony stimulating factor 2 receptor subunit beta
Gene Aliases
Beta common cytokine receptor; beta-GM-CSF receptor; betaGMR; CD131; CDw131; colony stimulating factor 2 receptor beta common subunit; colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) ; colony-stimulating factor-2 receptor, beta, low-affinity; cytokine receptor common subunit beta; GM-CSF/IL-3/IL-5 receptor common beta subunit; GM-CSF/IL-3/IL-5 receptor common beta-chain; IL3RB; IL5RB; interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3 receptor, beta (high affinity) ; SMDP5.
Gene ID
1439
Accession Number
NP_000386.1
Reactivity
Homo sapiens, Human
Target
Cytokine receptor common subunit beta
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CSF2RB ELISA Kit (Human) (OKEH08757) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CSF2RB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CSF2RB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CSF2RB captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85% (n = 20)
Sensitivity
0.123ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Cytokine receptor common subunit beta
NCBI Gene Symbol
CSF2RB
Protein Name
P32927
Gene Name URL
CSF2RB
Nucleotide Accession Number
NM_000395.2
Frequently Asked Questions
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