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ITPRID2 ELISA Kit (Mouse)

Selected Variant

OKEH08727-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

ITPR interacting domain containing 2

Gene Aliases

5730488C15Rik; CS; CS-; CS1; CS-1; ITPR-interacting domain-containing protein 2; K; ki-ras-induced actin-interacting protein; KRAP; mKIAA1927; protein ITPRID2; SPA; SPAG13; sperm specific antigen 2; sperm-specific antigen 2 homolog; Ss; Ssfa2.

Gene ID

70599

Accession Number

NP_542125.3

Reactivity

Mouse, Mus musculus

Target

Sperm-specific antigen 2 homolog

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ITPRID2 ELISA Kit (Mouse) (OKEH08727) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ITPRID2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ITPRID2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ITPRID2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

78-5000pg/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81% (n = 20)

Sensitivity

38pg/ml

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Protein ITPRID2

NCBI Gene Symbol

Itprid2

Protein Name

Q922B9

Gene Name URL

Itprid2

Nucleotide Accession Number

NM_080558.4

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