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GP1BB ELISA Kit (Human)

Selected Variant

OKEH08722-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Glycoprotein Ib platelet subunit beta

Gene Aliases

Antigen CD42b-beta; BDPLT1; BS; CD42C; glycoprotein Ib (platelet), beta polypeptide; glycoprotein Ib platelet beta subunit; GP-Ib beta; GPIBB; GPIbbeta; nuclear localization signal deleted in velocardiofacial syndrome; platelet glycoprotein Ib beta chain; platelet membrane glycoprotein Ib beta; truncated platelet membrane glycoprotein Ib beta.

Gene ID

2812

Accession Number

NP_000398.1

Reactivity

Homo sapiens, Human

Target

Platelet glycoprotein Ib beta chain

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology GP1BB ELISA Kit (Human) (OKEH08722) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GP1BB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GP1BB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GP1BB captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103% (n = 20)

Sensitivity

9.4pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Platelet glycoprotein Ib beta chain

NCBI Gene Symbol

GP1BB

Protein Name

P13224

Gene Name URL

GP1BB

Nucleotide Accession Number

NM_000407.4

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