What is the catalog number for SIRPA ELISA Kit (Mouse)?

The catalog number for SIRPA ELISA Kit (Mouse) is OKEH08720-96W.

What sizes are available for SIRPA ELISA Kit (Mouse)?

SIRPA ELISA Kit (Mouse) is available in the following size(s): 96 Tests.

Is SIRPA ELISA Kit (Mouse) in stock?

SIRPA ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

SIRPA ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Signal-regulatory protein alpha

Gene Aliases

AI835480; Bi; Bit; brain Ig-like molecule with tyrosine-based activation motifs; brain immunological-like with tyrosine-based motifs; CD172; CD172 antigen-like family member A; CD172a; Idd13.2; inhibitory receptor SHPS-1; mSIRP-alpha1; myD-1 antigen; P8; P84; protein tyrosine phosphatase, non-receptor type substrate 1; Pt; Ptpns1; SHP; SHP substrate 1; SHP-1; SHPS-1; signal-regulatory protein alpha-1; SIRP; sirp-alpha-1; tyrosine-protein phosphatase non-receptor type substrate 1.

Gene ID

19261

Accession Number

NP_001171118.1

Reactivity

Mouse, Mus musculus

Target

Tyrosine-protein phosphatase non-receptor type substrate 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SIRPA ELISA Kit (Mouse) (OKEH08720) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SIRPA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SIRPA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SIRPA captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol