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CKMT1B ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Creatine kinase, mitochondrial 1A, creatine kinase, mitochondrial 1B

Gene Aliases

Acidic-type mitochondrial creatine kinase; CKMT; CKMT1; creatine kinase U-type, mitochondrial; creatine kinase, mitochondrial 1 (ubiquitous) ; mia-CK; ubiquitous mitochondrial creatine kinase; U-MtCK; UMTCK.

Gene ID

1159, 548596

Accession Number

NP_001015001.1

Reactivity

Homo sapiens, Human

Target

Creatine kinase U-type, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CKMT1B, CKMT1A ELISA Kit (Human) (OKEH08700) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CKMT1B, CKMT1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CKMT1B, CKMT1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CKMT1B, CKMT1A captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

3.12-200U/L

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)

Sensitivity

1.54U/L

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Creatine kinase U-type, mitochondrial

NCBI Gene Symbol

CKMT1A, CKMT1B

Protein Name

P12532

Gene Name URL

CKMT1A, CKMT1B

Nucleotide Accession Number

NM_001015001.2

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