UNC5C ELISA Kit (Chicken)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Unc-5 netrin receptor C
Gene Aliases
CUNC-5H3; netrin receptor UNC5C; protein unc-5 homolog 3; protein unc-5 homolog C; unc-5 homolog 3; unc-5 homolog C; UNC5-like protein 3.
Gene ID
395101
Accession Number
NP_989782.1
Reactivity
Chicken, Gallus gallus
Target
Netrin receptor UNC5C
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology UNC5C ELISA Kit (Chicken) (OKEH08676) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UNC5C has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for UNC5C is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample UNC5C captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 113% (n = 20)
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Chicken Netrin receptor UNC5C
NCBI Gene Symbol
UNC5C
Protein Name
Q7T2Z5
Gene Name URL
UNC5C
Nucleotide Accession Number
NM_204451.1
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