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RDH16 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Retinol dehydrogenase 16

Gene Aliases

HRDH-E; human epidermal retinol dehydrogenase; Microsomal NAD (+) -dependent retinol dehydrogenase 4; microsomal NAD+-dependent retinol dehydrogenase 4; retinol dehydrogenase 16; retinol dehydrogenase 16 (all-trans and 13-cis) ; retinol dehydrogenase 16 (all-trans) ; RODH-4; SDR9C8; Short chain dehydrogenase/reductase family 9C member 8; short chain dehydrogenase/reductase family 9C, member 8; sterol/retinol dehydrogenase.

Gene ID

8608

Accession Number

NP_003699.3

Reactivity

Homo sapiens, Human

Target

Retinol dehydrogenase 16

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology RDH16 ELISA Kit (Human) (OKEH08666) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RDH16 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RDH16 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RDH16 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91% (n = 20)

Sensitivity

39.8pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Retinol dehydrogenase 16

NCBI Gene Symbol

RDH16

Protein Name

O75452

Gene Name URL

RDH16

Nucleotide Accession Number

NM_003708.4

Frequently Asked Questions

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