What is the catalog number for THEMIS ELISA Kit (Mouse)?

The catalog number for THEMIS ELISA Kit (Mouse) is OKEH08653-96W.

What sizes are available for THEMIS ELISA Kit (Mouse)?

THEMIS ELISA Kit (Mouse) is available in the following size(s): 96 Tests.

Is THEMIS ELISA Kit (Mouse) in stock?

THEMIS ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

THEMIS ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Thymocyte selection associated

Gene Aliases

E430004N04Rik; G; Gasp; grb2-associating protein; protein THEMIS; Protein thylex; signaling phosphoprotein specific for T cells; Spot; thylex; thymocyte selection pathway associated; thymocyte-expressed molecule involved in selection; thymus lymphocyte exclusively expressed; Ts; Tsepa.

Gene ID

210757

Accession Number

NP_001292592.1

Reactivity

Mouse, Mus musculus

Target

Protein THEMIS

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology THEMIS ELISA Kit (Mouse) (OKEH08653) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for THEMIS has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for THEMIS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample THEMIS captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol