What is the catalog number for H3-3B ELISA Kit (Bovine)?

The catalog number for H3-3B ELISA Kit (Bovine) is OKEH08624-96W.

What sizes are available for H3-3B ELISA Kit (Bovine)?

H3-3B ELISA Kit (Bovine) is available in the following size(s): 96 Tests.

Is H3-3B ELISA Kit (Bovine) in stock?

H3-3B ELISA Kit (Bovine) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

H3-3B ELISA Kit (Bovine)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

H3.3 histone A, H3.3 histone B

Gene Aliases

H3 histone, family 3A; H3 histone, family 3B; H3.3B; H3-3B; H3F3A; H3F3B; histone H3.3.

Gene ID

326601, 617051

Accession Number

NP_001014411.1

Reactivity

Bos taurus, Bovine

Target

Histone H3.3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology H3-3B, H3F3B ELISA Kit (OKEH08624) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-H3-3B, H3F3B antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated H3-3B, H3F3B and incubated. The H3-3B, H3F3B found in the sample or standards competes with the biotinylated H3-3B, H3F3B for limited binding sites on the immobilized anti-H3-3B, H3F3B antibody. Excess unbound biotinylated H3-3B, H3F3B and sample or standard H3-3B, H3F3B is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated H3-3B, H3F3B captured in the well and inversely proportional to the amount of H3-3B, H3F3B which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol