IMMP2L ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Inner mitochondrial membrane peptidase subunit 2
Gene Aliases
IMMP2L-IT1; IMP2; IMP2 inner mitochondrial membrane peptidase-like; IMP2 inner mitochondrial membrane protease-like; IMP2-LIKE; IMP2-like protein; inner mitochondrial membrane peptidase 2 like; mitochondrial inner membrane protease subunit 2.
Gene ID
83943
Accession Number
NP_001231535.1
Reactivity
Homo sapiens, Human
Target
Mitochondrial inner membrane protease subunit 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology IMMP2L ELISA Kit (OKEH08603) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-IMMP2L antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated IMMP2L and incubated. The IMMP2L found in the sample or standards competes with the biotinylated IMMP2L for limited binding sites on the immobilized anti-IMMP2L antibody. Excess unbound biotinylated IMMP2L and sample or standard IMMP2L is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated IMMP2L captured in the well and inversely proportional to the amount of IMMP2L which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98% (n = 20)
Sensitivity
0.26ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Mitochondrial inner membrane protease subunit 2
NCBI Gene Symbol
IMMP2L
Protein Name
Q96T52
Gene Name URL
IMMP2L
Nucleotide Accession Number
NM_001244606.1
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