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NUS1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

NUS1 dehydrodolichyl diphosphate synthase subunit

Gene Aliases

C6orf68; CDG1AA; cis-prenyltransferase subunit NgBR; dehydrodolichyl diphosphate syntase complex subunit NUS1; dehydrodolichyl diphosphate synthase complex subunit NUS1; di-trans, poly-cis-decaprenylcistransferase; MGC:7199; MRD55; NgBR; Nogo-B receptor; nuclear undecaprenyl pyrophosphate synthase 1 homolog; TANGO14; transport and golgi organization 14 homolog.

Gene ID

116150

Accession Number

NP_612468.1

Reactivity

Homo sapiens, Human

Target

Dehydrodolichyl diphosphate synthase complex subunit NUS1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NUS1 ELISA Kit (Human) (OKEH08597) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NUS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NUS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NUS1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81% (n = 20)

Sensitivity

0.397ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Dehydrodolichyl diphosphate synthase complex subunit NUS1

NCBI Gene Symbol

NUS1

Protein Name

Q96E22

Gene Name URL

NUS1

Nucleotide Accession Number

NM_138459.3

Frequently Asked Questions

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