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NUDT11 ELISA Kit (Human)

Selected Variant

OKEH08594-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Nudix hydrolase 11

Gene Aliases

APS1; ASP1; diadenosine 5',5'''-P1, P6-hexaphosphate hydrolase 3-beta; diadenosine hexaphosphate hydrolase (AMP-forming) ; diphosphoinositol polyphosphate phosphohydrolase 3, beta; diphosphoinositol polyphosphate phosphohydrolase 3-beta; DIPP3b; DIPP3beta; DIPP3-beta; DIPP-3-beta; hAps1; hDIPP3beta; nucleoside diphosphate-linked moiety X motif 11; nudix (nucleoside diphosphate linked moiety X) -type motif 11; nudix motif 11.

Gene ID

55190

Accession Number

NP_060629.2

Reactivity

Homo sapiens, Human

Target

Diphosphoinositol polyphosphate phosphohydrolase 3-beta

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NUDT11 ELISA Kit (Human) (OKEH08594) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NUDT11 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NUDT11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NUDT11 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98% (n = 20)

Sensitivity

22pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Diphosphoinositol polyphosphate phosphohydrolase 3-beta

NCBI Gene Symbol

NUDT11

Protein Name

Q96G61

Gene Name URL

NUDT11

Nucleotide Accession Number

NM_018159.3

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