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CD200R1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

CD200 receptor 1

Gene Aliases

CD200 cell surface glycoprotein receptor; CD200R; cell surface glycoprotein CD200 receptor 1; cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; HCRTR2; MOX2 receptor; MOX2R; OX2R.

Gene ID

131450

Accession Number

NP_620161.1

Reactivity

Homo sapiens, Human

Target

Cell surface glycoprotein CD200 receptor 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CD200R1 ELISA Kit (Human) (OKEH08586) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD200R1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD200R1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD200R1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% (n = 20)

Sensitivity

0.085ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Cell surface glycoprotein CD200 receptor 1

NCBI Gene Symbol

CD200R1

Protein Name

Q8TD46

Gene Name URL

CD200R1

Nucleotide Accession Number

NM_138806.3

Frequently Asked Questions

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