ARL13B ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
ADP ribosylation factor like GTPase 13B
Gene Aliases
ADP-ribosylation factor-like 13B; ADP-ribosylation factor-like 2-like 1; ADP-ribosylation factor-like protein 13B; ADP-ribosylation factor-like protein 2-like 1; ARL2L1; ARL2-like protein 1; JBTS8.
Gene ID
200894
Accession Number
NP_001167621.1
Reactivity
Homo sapiens, Human
Target
ADP-ribosylation factor-like protein 13B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology ARL13B ELISA Kit (OKEH08582) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-ARL13B antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ARL13B and incubated. The ARL13B found in the sample or standards competes with the biotinylated ARL13B for limited binding sites on the immobilized anti-ARL13B antibody. Excess unbound biotinylated ARL13B and sample or standard ARL13B is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ARL13B captured in the well and inversely proportional to the amount of ARL13B which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)
Sensitivity
0.134ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human ADP-ribosylation factor-like protein 13B
NCBI Gene Symbol
ARL13B
Protein Name
Q3SXY8
Gene Name URL
ARL13B
Nucleotide Accession Number
NM_001174150.1
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