PLEKHG6 ELISA Kit (Human)

Product Specifications

Reproducibility

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CAS Number

7732-18-5

Gene Name

Pleckstrin homology and RhoGEF domain containing G6

Gene Aliases

MyoGEF; myosin II interacting GEF; myosin interacting guanine nucleotide exchange factor; Myosin-interacting guanine nucleotide exchange factor; PH domain-containing family G member 6; pleckstrin homology domain containing, family G (with RhoGef domain) member 6; pleckstrin homology domain-containing family G member 6.

Gene ID

55200

Accession Number

NP_001138328.1

Reactivity

Homo sapiens, Human

Target

Pleckstrin homology domain-containing family G member 6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PLEKHG6 ELISA Kit (Human) (OKEH08581) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PLEKHG6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PLEKHG6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PLEKHG6 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol