What is the catalog number for DNAJC27 ELISA Kit (Chicken)?

The catalog number for DNAJC27 ELISA Kit (Chicken) is OKEH08573-96W.

What sizes are available for DNAJC27 ELISA Kit (Chicken)?

DNAJC27 ELISA Kit (Chicken) is available in the following size(s): 96 Well.

Is DNAJC27 ELISA Kit (Chicken) in stock?

DNAJC27 ELISA Kit (Chicken) is currently made to order / not held in immediate stock. Contact us for lead time.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

DNAJC27 ELISA Kit (Chicken)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

DnaJ heat shock protein family (Hsp40) member C27

Gene Aliases

DnaJ (Hsp40) homolog, subfamily C, member 27; dnaJ homolog subfamily C member 27; rab and DnaJ domain containing; rab and DnaJ domain-containing protein; RBJ.

Gene ID

402783

Accession Number

NP_998723.1

Reactivity

Chicken, Gallus gallus

Target

DnaJ homolog subfamily C member 27

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DNAJC27 ELISA Kit (Chicken) (OKEH08573) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DNAJC27 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DNAJC27 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DNAJC27 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% (n = 20)

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Chicken DnaJ homolog subfamily C member 27

NCBI Gene Symbol

DNAJC27

Protein Name

Q6IMM1

Gene Name URL

DNAJC27

Nucleotide Accession Number

NM_213558.1

Frequently Asked Questions

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