CLEC12A ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
C-type lectin domain family 12 member A
Gene Aliases
CD371; CLL1; CLL-1; C-type lectin domain family 12 member A; C-type lectin protein CLL-1; C-type lectin-like molecule 1; C-type lectin-like molecule-1; DCAL-2; dendritic cell-associated lectin 2; MICL; myeloid inhibitory C-type lectin-like receptor.
Gene ID
160364
Accession Number
NP_001193939.1
Reactivity
Homo sapiens, Human
Target
C-type lectin domain family 12 member A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CLEC12A ELISA Kit (Human) (OKEH08572) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLEC12A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLEC12A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLEC12A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96% (n = 20)
Sensitivity
0.067ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human C-type lectin domain family 12 member A
NCBI Gene Symbol
CLEC12A
Protein Name
Q5QGZ9
Gene Name URL
CLEC12A
Nucleotide Accession Number
NM_001207010.1
Frequently Asked Questions
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