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CLEC12A ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

C-type lectin domain family 12 member A

Gene Aliases

CD371; CLL1; CLL-1; C-type lectin domain family 12 member A; C-type lectin protein CLL-1; C-type lectin-like molecule 1; C-type lectin-like molecule-1; DCAL-2; dendritic cell-associated lectin 2; MICL; myeloid inhibitory C-type lectin-like receptor.

Gene ID

160364

Accession Number

NP_001193939.1

Reactivity

Homo sapiens, Human

Target

C-type lectin domain family 12 member A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CLEC12A ELISA Kit (Human) (OKEH08572) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLEC12A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLEC12A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLEC12A captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96% (n = 20)

Sensitivity

0.067ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human C-type lectin domain family 12 member A

NCBI Gene Symbol

CLEC12A

Protein Name

Q5QGZ9

Gene Name URL

CLEC12A

Nucleotide Accession Number

NM_001207010.1

Frequently Asked Questions

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